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This study examines the relationships among twelve manuscripts in Acts and demonstrates that, while all share a certain number of readings, several of them comprise Family 1739 in Acts. The study utilizes quantitative and profile analyses to demonstrate the family’s existence and to describe it in detail. The most representative manuscripts of the family have close affinities with the Alexandrian text of Acts; other manuscripts, although exhibiting a closer relationship with the Byzantine text of Acts in general, share a significant number of Family 1739 readings. Included in the study are the results of the statistical research and most of the readings in Acts that informed the conclusions.